荧光修饰
多肽荧光修饰 - 染料和猝灭剂
AMC (7-Amino-4-methyl-coumarin)
UV-excitable dye, used in enzyme assays using cuvettes or flow cytometry.
Cy3, Cy5
Cy3，Cy5是具有极高消光系数和荧光的染料。因此，它们特别适合于细胞中肽的非常灵敏的定位测定。它们的缺点是分子在
多肽合成条件下的高度不稳定性。因此，产率相对较低。
Dabcyl
Dabcyl is a non-fluorescent dye predominantly used as a quencher for other fluorophores (esp.
Fluorescein type dyes, EDANS.).
If Dabcyl is coupled to a peptide in close proximity to a fluorophore, it absorbs the emitted light of the
fluorophore. Enlarging this distance (i.e. by enzymatic cleavage of the peptide) results in excitation of
the fluorophore with an emission signal that can be detected.
Dansyl
Dansyl is also used as a fluorophore quencher. Unlike Dabcyl, it inherits own fluorescence and thus
might not be as useful for highly sensitive assays.
2,4-Dinitrophenyl (DNP)
2,4-Dinitropheny is a non-fluorescent dye that can be used as a fluorophore quencher (see Dabcyl for more details).
DNP-Lysine
DNP-Lys是一种非荧光染料，可以用作荧光淬灭剂，用于
多肽定制（参考Dabcyl）
EDANS (5-((2-aminoethyl)amino)napthalene-1-sulfonic acid)
EDANS is a commonly used dye in FRET (fluorescence resonance energy transfer) peptides in combination with Dabcyl as quencher.
Fluorescein
Fluorescein is the commonly used fluorescent dye in confocal laser-scanning microscopy and flow cytometry applications.
NBD (7-nitrobenz-2-oxa-1, 3-diazole)
NBD is a fluorescent dye, used for amine modification.
p-Nitro-Aniline
p-Nitro-Aniline is a chromogen used as colorimetric enzyme substrate in many standard enzyme assays in cuvettes.
Rhodamine B
Rhodamine B represents one among a numerous range of rhodamine dyes, used in
fluorescent peptide assays.
Tamra
Tamra is the most commonly used rhodamine dye in fluorescence assays.
